Ungayihlanza kanjani isidlo sesiko seseli lapho usisebenzisa

I-Cell Culture Dish (150mm): Idizayini ekhethekile yokuthuthukisa ukuthuthuka yandisa amandla wesembozo futhi phansi kwesitsha, ithuthukisa ukukhuluphala ngasikhathi sinye, futhi yandisa i-adsorption yodonga lwangaphakathi lwesitsha sesiko.

Ukuhlanza izinyathelo zezitsha zesiko leseli:

Ngokuvamile, kuya ezitebhisini ezine zokuphamba, ukuklwebheka, ukukha, nokuhlanza.

cell culture dish

1. Ukufaka: I-Glassware entsha noma esetshenzisiwe kufanele ifakwe emanzini okokuqala ukuthambisa futhi ichithe okunamathiselwe. I-Glassware entsha kufanele ivele ihluthwe ngamanzi ompompi ngaphambi kokusebenzisa, bese ifakwe emanzini ubusuku ngo-5% i-hydrochloric acid; I-Glassware esetshenzisiwe ivame ukuba namaprotheni amaningi namafutha anamathele kuwo, okungekhona lula ukugeza ngemuva kokomiswa, ngakho-ke kufanele kufakwe emanzini ahlanzekile ngokushesha ngemuva kokuklama.

2. Ukukhahlela: Beka ingilazi efakwe emanzini emanzini okuhlanza bese uklebhula kaningi ngebhulashi elithambile. Ungashiyi isikhala esifile futhi uvikele ukulimala kokunye kokunye kwezinto. Geza bese wemisa i-glassware ehlanzekile ukuze ubheke.

3. UKUKHANYA: UKUTHENGA KUSUKA IZIMALI ezichazwe ngenhla kwikhambi lokuhlanza, eyaziwa nangokuthi isixazululo se-acid, ukususa izinto ezisele ezisele ebusweni bezitsha ze-oxidation eziqinile zekhambi le-acid. I-Pickling akufanele ibe ngaphansi kwamahora ayisithupha, imvamisa ubusuku bonke noma ngaphezulu. Qaphela izitsha.

4. Hlambulula: Izitsha ngemuva kokukhahlela nokukhamba kumele zihlatshwe ngokuphelele ngamanzi. Ukuthi izitsha zihlanzwa ngemuva kokudonsa kuzothinta ngqo impumelelo noma ukwehluleka kwesiko leseli. Hlanza ngesandla izitsha ngemuva kokukhamba, futhi izitsha ngakunye kufanele ziphindaphinde "okungenani amanzi" okungenani amahlandla ayi-15, bese ekugcineni ziyiphonsa ngamanzi ancibilikisiwe amahlandla amahlandla ama-2-3, womile noma akulume ukuze akwazi ukukusebenzisa kamuva.