Ukuphathwa kwe-TC kwezitsha zesiko leseli kuyisinyathelo esibaluleke kakhulu sokuqinisekisa imiphumela efanelekile, ethembekile neyakhiwa kabusha ngesikhathi sesiko leseli. Le ndatshana izochaza inhloso, indlela, kanye nemikhuba emihle kakhulu yokukhohlisa kwe-TC futhi uxoxe ngokubaluleka kwayo ngocwaningo lwesiko leselula. 1. Inhloso yokusebenza kwe-TC Ukwelashwa kwe-TC (owaziwa nangokuthi ukwelashwa kwesiko lezicubu) ukwelashwa okukhethekile kwezitsha zesiko leseli ukuthuthukisa amandla wazo angaphezulu, vikela ukunamathela kweseli okumpofu noma ukuguquguquka ngokweqile, ngaleyo ndlela kwandise ukufana kokunamathiselwe kweseli. Ukwelashwa kwe-TC kungenza amaseli anamathele kangcono phansi kwesitsha sesiko, futhi angakhuthaza ukukhula kwamaselula nokuhlukaniswa kwamaselula, kanti futhi anciphisa ubungozi bokufa kweseli ngenxa yokunamathela kabi ngenxa yokunamathela kabi. 2. Indlela yokucubungula ye-TC Kunezindlela ezahlukahlukene zokwelashwa kwe-TC, kufaka phakathi izindlela zomzimba nezamakhemikhali. Phakathi kwazo, indlela ebonakalayo ukunciphisa ukungezwani kwesiko lesiko ngesivinini esikhulu sengxabano yokunciphisa i-ejenti ukukhuthaza ukunamathiselwa kweseli; Indlela yamakhemikhali ukusebenzisa izinto ezifana ne-siloxosane ukumboza ingaphezulu lesitsha sesiko ukuthuthukisa ukuntuleka komhlaba. 3. Umkhuba Omuhle Wokusebenza kwe-TC Lapho wenza ukwelashwa kwe-TC, amaphuzu alandelayo kufanele aqashelwe: 3.1 Ukuhlanza: Ngaphambi kokuthatha isidlo se-petri ukuze ukwelashwa kwe-TC, kufanele kuqinisekiswe ukuthi indawo yayo ihlanzekile, iyinyumba futhi yomile. Lokhu kungatholakala ngokusebenzisa i-organic solvent (njenge-ethanol noma i-methanol) kanye nesibani se-UV. 3.2 Isikhathi: Isikhathi sokucubungula i-TC kufanele silawulwe ngokunembile. Izinto ezihlukile zesiko lezinto zokwelashwa nezinhlobo zokwelashwa kungadinga izikhathi zokwelashwa ezahlukahlukene. Ngakho-ke, ngaphambi kokwenza ukwelashwa kwe-TC, kufanele ubheke imiyalo noma izincwadi ezinikezwe umphakeli, futhi wenze izinguquko ezifanele ngokwezidingo zokuhlola. I-3.3 Ukulawulwa Kwekhwalithi: Umphumela wokwelashwa kwe-TC uzothinta imiphumela esezingeni elilandelayo leselula. Ukuze uqinisekise ukuthi i-batch ngayinye yezitsha ze-petri ingathola umphumela we-TC ongaguquki, ukuhlolwa okuphindaphindiwe kanye nokulawulwa kwekhwalithi kungenziwa ukuqinisekisa ukungaguquguquki kokuphazamiseka komhlaba ngamunye wezitsha ze-petri. 4. Ukubaluleka kokwelashwa kwe-TC kocwaningo lwesiko leseli Ukwelashwa kwe-TC kungathuthuki nje kuphela ukufana kokunamathiselwe kwamaseli kanye nezinga lokukhula, kepha kwanciphisa nokufa kweseli nokukhuthaza ukuhlukaniswa kweseli. Lezi zinzuzo zingadlala indima ebalulekile kuzivivinyo eziningi zesiko leseli, njenge-cell forliferation, ukuhlolwa kwezidakamizwa, nokuhlolwa kwe-cytotoxity, njll.
