Izinkinga zokuvalwa kwezimpawu nezinkinga zokungcola kumapuleti ama-Multiwell Cell Culture Cultes

Iyalayisha nokusingathwa kwamapuleti ama-stude wesiko ahlukahlukene:

Ipuleti lesiko leseli nalo lilandela umgomo we-ASEPSIS aqinile lapho wenza imisebenzi yeseli. Yonke imisebenzi kumele ihlelwe futhi yesayensi, futhi ngeke ibange umthelela owengeziwe ekukhuleni kwamaseli. Inkinga evame kakhulu yindlela yokuqinisekisa ukufana kwamangqamuzana ngemuva kokungeza isampula futhi unciphise umthelela wokushintsha okuphakathi kwesimo sokukhula kwamaseli.

Buza:
Amabhodlela angamapuleti angama-96 nama-24 - amapuleti amasiko noma izitsha ze-petri akhululekile kakhulu, okulungele umoya omncane, kepha kuzokwehla kwamagciwane, kodwa futhi kuzokwehla kwama-bacterium?

Impendulo:
Isembozo sikhululekile kakhulu, esingesiko elivulelekile, futhi inhloso yalokhu ukungena umoya (eqinisweni, ukwenza i-CO2 ngaphandle kwesitsha sesiko elishintshana ngokugcwele nesiko lesiko Medium).
Yonke into inezinzuzo nobubi, okuyinto ekhulisa amathuba okungcola. Ngaphezu kwalokho, lokhu kubangela uketshezi esitsheni ukuhwamuka, okuphawulekayo ngokuthola izidakamizwa eziqondile. Ngakho-ke, lezi zinyathelo ezimbili ezilandelayo ziyadingeka: a. Umoya okwi-incubator kumele uhlanzeke (ukukhanya okujwayelekile kwe-ultraviolet, ukuklulwa kotshwala, futhi i-incubator kufanele ivulwe futhi ivalwe kancane ngangokunokwenzeka) b. Umswakama ku-incubator kumele uhlale ugcinwe ngo-100% (i-sink enamanzi ansundu afakwe emanzini afakwe ku-incubator).
Njengesitsha se-petri, futhi siyisitsha esinezimbobo ezibheke phezulu, futhi ngeke zingcoliswe. Kakhulu ngenxa yengcindezi engemihle ye-airflow ekhiqizwe umphetho we- "L" wesembozo, kukhona ama-microorganisms anamathele othulini, futhi uthuli oluphathwe yi-airflow alukwazi ukudlula emaphethelweni embobo ekhiqiza ingcindezi engemihle. Umphumela wokungena komoya uwukuphazanyiswa komoya kuphela, futhi akukho ukugeleza komoya okuzokwenziwa, ngakho-ke kuzomane uphefumule futhi ungangeni kabusha futhi ungangeni kabusha.

Buza:
Usebenzisa ipuleti elinezinkulungwane ezingama-24, kunemisebenzi kweminye yemithombo (ebhentshini elihlanzekile elihlanzekile), futhi kukhona amaseli azokwakhiwa kweminye imithombo. Ngikhathazekile ukuthi lokhu kuzodala ukungcola. Angizi ukuthi yini okufanele nginake?

Impendulo:
Uma ukuhlinzwa kulinganiswa ebhentshini elihlanzekile, kufanele kube kulungile. Ngicabanga ukuthi ungasebenzisa ngokugcwele isembozo sepuleti lesiko, zama ukudalula kuphela izimbobo ezizosetshenziswa, futhi zimboze ezinye izimbobo ezinezembozo.
Zibheke ngokuphelele ngaphambi kokusebenzisa futhi zisebenzise ngokugcwele zonke izimbobo; Uma udinga ukusebenzisa izimbobo ezimbalwa kuphela, ungasebenzisa uhlangothi olulodwa kuphela, bese umboza abanye ngesembozo. Ngijwayele ukusebenzisa umgodi ofanele kuqala (kulula ukwengeza amasampula esandleni sokudla).

Lapho usebenza, sebenzisa ama-slides ambalwa engilazi ukuze ukhulise uhlangothi olulodwa lwebhodi, ungavuli isembozo ngokuphelele, ngokuvamile akunankinga.
Ukusatshalaliswa Kweseli Okungalingani Nezixazululo

Buza:
Amangqamuzana afakwe kupuleti lesiko, futhi amangqamuzana ahlala ahlangana engxenyeni ye-peripheral, indlela yokubhekana nayo?

Impendulo:
Ngingabuza ukuthi amangqamuzana akho ahlanganiswa kanjani? Ingabe i-Pipetting noma inikina ipuleti lesiko? Uma kungokokugcina, futhi uma kunyakaziswa embuthanweni, kungenzeka ukuthi amaseli azophonswa engxenyeni ye-peripheral ngenxa yama-centrifugal Force, okuholela emangqamu ambalwa phakathi. Ngaphezu kwamasonto amane!
Nansi indlela enhle: Ngaphambi kokuhlakulela ipuleti lembewu, faka isiko lepuleti ku-incubator amahora ambalwa wokugcwaliswa bese uyikhipha. Amandla kufanele abe ukukhanya lapho etshala amaseli. Ukungeza kancane kuvumela ukumiswa kweseli ukugeleza emithonjeni yepuleti, futhi amangqamuzana amasiko akhula ngokulinganayo. Khumbula ukungalokothi unyakaze nge-shaker, noma amaseli akho azohlangana njengoba ushilo.
Uma kuncane ububanzi bomgodi wepuleti lesiko, le nto isobala kakhulu. Le nto ayinakugwenywa amapuleti angama-24 kuya kwangama-96, ngoba i-liquid inamathela odongeni ukuze isiko lesiko likhathele alwenzele izinga loketshezi, kepha i-perifery iphakeme, njengesibuko esiphakeme. Kodwa-ke, lapho usebenzisa lezi zinhlobo ezimbili zamapuleti e-orifice, akunakwenzeka ukwengeza inani elanele lesisombululo sesiko ngenxa yesidingo sokungenelela okwengeziwe, ukuze amaseli avele "kanye nesixazululo sesiko. Kuya ngokuthi yiziphi izinkomba okudingeka uzigcine. Uma kunguMTT, iMmunonistochemistry izothinta imiphumela ngenxa yokufaka iseli.
Lapho ukugaya amaseli, naka ama-Pipetting ngokulinganayo ukugwema ama-clumps weselula, kanye nenani lesisombululo sesiko emthonjeni kufanele anele. Ngokuvamile, engeza ikhambi elanele lesiko lapho ufaka i-ioculise, bese ushintsha ikhambi kanye lapho ungeza ukungenelela. Inani lesixazululo sesiko elingezwe kwikhambi lokungenelela lilingana nenani lesisombululo sesiko ngesikhathi sokungena, kulokhu, into "esethiwe" izokwenzelwa.

Buza:
Engikwenzile kwakuwukuhlola ukwakheka kwe-plaque. Kungcono ukusakaza amaseli engxenyeni efanayo. Iningi lezimbobo lihamba kahle lapho leli gciwane libodwa, kanti abanye babo abafani. Kunokwehluka okukhulu eqenjini leselula. Ngingathanda ukukubuza ukuthi iyiphi indlela oyisebenzisayo lapho ungeza amaseli?

Impendulo:
Kubalulekile kuma-pipete amaseli ekumisweni kweseli elilodwa ngemuva kokugaya! Qiniseka ukuthi inani lamaseli emthonjeni ngamunye liyaguquguquka lapho lihlukanisa ipuleti!
Vele, kukhona nezinto ezicubungula. Ukufana phakathi kwemithombo nakho kubaluleke kakhulu. Isibonelo, lapho wengeza umuthi, hlanganisa nomuthi ngemuva kokujabula ukuze uqinisekise ukuthi ukugcwaliseka komthombo ngamunye kuvumelana!
Ngaphezu kwalokho, lapho ungeza amaseli nezidakamizwa, iqembu lokuhlola kanye neqembu elilawulayo kufanele lingezwe ukuze kugwenywe umehluko ekubumbeni kwamaseli nokuhlushwa kwezidakamizwa okubangelwa ukulandelana kwamasampula!

Buza:
Ukuqhuma sekuvele umfaniswano, kepha amapulangwe, izimbobo ezi-6, nezimbobo ezingama-24 zihlala zinganele ngandlela thile, futhi zigxile kancane phakathi. Ngaphezu kwalokho, kusobala ukuthi ukubala kwenziwa, futhi ngemuva kosuku noma ezimbili, ubuningi bomgodi ngamunye buhlukile, okuzothinta ukutholwa. Ingabe ikhona indlela enhle?

Impendulo:
Uma usebenzisa i-pasteur pipette, ungamunca kakhulu isikhathi ngasinye, ngoba amaseli e-pipette azocwila ngokuzenzakalelayo, imvamisa inani lamaseli ezimbonini ezimbalwa zokuqala likhulu, futhi ukumiswa kweseli kuvame ukufana Landela umzila we-S-shape.
Uma usebenzisa i-pipette, amathiphu kufanele acutshungulwe futhi asuswe ukuze alimaze amaseli, ukuze kuthathwe isikhathi ngasinye lapho uketshezi luthambekele emthonjeni. Kunembe kakhulu ukwengeza umgodi owodwa kuya-munye ngethiphu. Kepha futhi naka unaka ukuxuba ukumiswa.

Ukusetha iqembu elihambisanayo, n kumele kube kukhulu ngokwanele (ungakubala).

Musa ukuzamazama ngemuva kokuzamazama, njengoba ukuzamazama kuzokwenza amaseli ukuba ahlanganise esikhungweni. Kuhle kakhulu ukunamathela kanye.

I-Porous Cell Culture Culture Plate Yongyue Medical Condical ilokhu ibhekele ukulawulwa kwekhwalithi njengempilo yebhizinisi, futhi yalandela ukuthuthuka okuqhubekayo kokuncintisana kwebhizinisi. Inkampani ihlala inamathela emoyeni wamabhizinisi wokulalela imithetho nemigomo, ukuba ngokuqinile nokuzikhuthazela, futhi nesibindi sokuthatha umthwalo wemfanelo njengesezingeni eliphezulu kanye nesevisi enhle kakhulu, kanye nokuhlangana ne Izidingo zamakhasimende ngezinga elikhulu kakhulu. . Win-Win Namakhasimende inhloso yethu yentuthuko. Yongyue Medical! Umlingani wakho othembekile. Sizimisele ukubambisana ngokweqiniso nani ukudala ikusasa elingcono.