Yini okufanele inakeke lapho usebenzisa izitsha zikaPetri?

Isitsha se-petri ngumkhumbi welabhoratri osetshenziselwa ukukhulisa ama-microorganisms noma amasiko weseli. Siqukethe isisekelo esisezingeni eliphakeme se-disc nesembozo. Izinto zesitsha se-petri zihlukaniswe ngezigaba ezimbili, ikakhulukazi ipulasitiki nengilazi. Izitshalo zezitshalo, amasiko ama-microbial, namasiko anamathiselwe amangilazi wamaseli ezilwane nawo asetshenziswa kakhulu. Ipulasitiki yenziwe nge-polyethylene futhi ilungele i-inoculation yaselebhu, i-inoculation kanye nokuhlukaniswa kwamagciwane kanye nokuthola izingodo zezitshalo.

Yasungulwa ekuqaleni ngo-1887 nge-bacteriologist uJulius Richard Pelri (1852-1921) Ngaphansi kwesazi sebhayisoloji saseJalimane uRobert Koch, ngakho-ke kwaziwa nangokuthi "uNkulunkulu". Isitsha se-Petri "sikaLi"

1. Ukugeza izitsha ze-petri

a) Ukufaka: I-Glassware entsha noma esetshenzisiwe kufanele ifakwe emanzini okokuqala ukuthambisa futhi ichithe okunamathiselwe. I-Glassware entsha idinga kuphela ukuklanywa ngamanzi ompompi ngaphambi kokusebenzisa, bese ifakwe emanzini angu-5% we-hydrochloric ngobusuku obu-5; I-Glassware esetshenzisiwe imvamisa inamaprotheni amaningi namafutha anamathele kuwo, okungekhona lula ukugeza ngemuva kokomiswa. Ngakho-ke, kufanele ifakwe emanzini ahlanzekile bese iklabha ngokushesha ngemuva kokusetshenziswa.

b) Ukukhahlela: Beka ingilazi efakwe emanzini emanzini okugeza izitsha, bese uyikhahlela kaningi ngebhulashi elithambile. Ungashiyi isikhala esifile futhi uvikele ukulimaza ukuqeda okungaphezulu kohlelo lokusebenza. Geza bese wemisa i-glassware ehlanzekile ukuze ubheke.

c) Ukukhalela: Ukudonsa ukucwilisa izimpahla ezichazwe ngenhla kwisixazululo sokuhlanza, esaziwa nangokuthi isixazululo se-acid, ukususa izinto ezingase zihlale ebusweni be-oxidation eqinile ngenxa ye-oxidation eqinile yekhambi le-acid. I-Pickling akufanele ibe ngaphansi kwamahora ayisithupha, imvamisa ubusuku bonke noma ngaphezulu. Qaphela izitsha.

d) Ukuhlanza: I-Tableware kumele ihlatshwe ngokugcwele ngamanzi ngemuva kokukhahlela nokubala. Ukuthi izitsha zihlanzwa ngemuva kokuthatha ngokuqondile zithinta impumelelo noma ukwehluleka kwesiko leseli. Geza ngesandla izitsha ezinamakhemikhali. I-Utensil ngayinye kufanele iphinde "ikhishwe amanzi" okungenani amahlandla ayi-15, futhi ekugcineni yageza izikhathi ezingama-2-3 ngamanzi ancibilikisiwe aphindwe kabili, omisiwe noma omisiwe futhi agcwele ukusetshenziswa kamuva.

e) inzalo: Izitsha zeplastiki ezilahlekile zepulasitiki zivame ukufakwa inzalo ngokulandelana noma inzalo yamakhemikhali lapho zishiya ifektri.

2. Ukuhlukaniswa kwezitsha ze-PETRI

a) Ngokuya ngokusetshenziswa okuhlukile kwesitsha se-petri, kungahlukaniswa ngesitsha sesiko leseli kanye nesitsha sesiko lamagciwane.

b) Ngokusho kwezinto ezihlukile zokukhiqiza, ihlukaniswe izitsha zePetri Petri nezitsha ze-Petri, kepha zombili izitsha ze-petri ezingenisiwe kanye nezitsha ze-petri ezilahlwayo zenziwa ngezinto zokwakha zepulasitiki.

c) Ngokusho kosayizi abahlukahlukene, kungavame ukuhlukaniswa izitsha ze-petri ngamawazi angama-35mm, 60mm, 90mm naku-150mm.

d) Ngokusho kwezingxenye ezahlukahlukene, ingahlukaniswa izitsha ezihlukaniswe ngama-petri ezihlukanisiwe ezi-2, izitsha ezihlukaniswe ngama-3, njll.

e) okuqukethwe kwesitsha sePetri kuhlukaniswe ngezigaba ezimbili, ikakhulukazi ipulasitiki nengilazi, futhi ingilazi lingasetshenziselwa izinto zezitshalo, amasiko ama-microbial kanye nesiko lokunamathela kwezilwane zezilwane. Amapulasitiki epulasitiki angahle enziwe nge-polyethylene, futhi kukhona okuchithwa futhi okusetshenziswayo okuningana, okulungele ukungena kwelabhoratri, ukufuya, nokuhlukaniswa kwamagciwane, futhi kungasetshenziselwa ukutshala izinsiza zezitshalo.

3. Izindaba ezidinga ukunakwa ekusetshenzisweni kwezitsha zesiko

a) Ngemuva kokuhlanza kanye nokubulala amagciwane ngaphambi kokusebenzisa, ukuthi ngabe isidlo se-petri sihlanzekile noma cha kunethonya elikhulu emsebenzini, okungathinta i-PH ye-Medium. Uma kunamakhemikhali athile, kuzovimba ukukhula kwamagciwane.

b) Izitsha ezisanda kuthengwa ze-PETRI kufanele zihlatshwe ngamanzi ashisayo kuqala, bese zifakwe esikhaleni se-hydrochloric acid ngengxenye ye-1% noma i-2% ukuze kususwe ama-alkaline ama-alkaline, bese ukhanyisa kabili ngamanzi ancishisiwe.

c) Uma ufuna ukuhlakulela ama-bacteria, sebenzisa umusi wengcindezelo ephezulu (ngokuvamile 6.8 * 10 kuya kumandla wesi-5 we-PA High-Pressure Steam), abilayo ku-120 ° C imizuzu engama-30, noma abile ngokushisa egumbini lokushisa , okungukuthi, beka isidlo se-petri sikubeke kuhhavini futhi ugcine amazinga okushisa acishe abe ngu-120 ° C amahora ama-2 ukubulala amaseli athelelekile.

d) Izitsha ze-petri ezingcolile kuphela ezingasetshenziselwa i-inoculation namasiko.