Ukukhetha kanye nokusetshenziswa kwamapuleti ama-Cell Culture

Njengethuluzi elibalulekile elivame ukusetshenziswa emasikweni weseli, amapuleti ama-cell Culture anezimo ezahlukahlukene, ukucaciswa kanye nokusebenzisa.

Ngabe udidekile futhi ngokukhetha ipuleti lesiko elifanele?

Angazi ukuthi usebenzise kanjani ipuleti lesiko elifanele futhi kahle?

Ngabe udideke nokuthi ungayisebenzisa kanjani iBhodi Yokuqeqesha?

Okulandelayo, yisikhathi sokuzijwayeza ngohlelo lokusebenza lokufunda.

Ukuhlukaniswa kwamapuleti ama-Cell Culture

1) Amapuleti amasiko wezicubu angahlukaniswa ngezinga eliphansi kanye nelithole eliphansi (okwakheke kakhulu futhi okubunjiwe kwe-V) ngokuya ngesimo esingezansi;

2) Inani lemithombo yamasiko lingu-6, 12, 24, 48, 96, 384, 1536 Wells, njll;

3) Kuya ngokoqobo, kukhona amapuleti aseTerasaki namapuleti ajwayelekile wesiko. Ukukhetha kuncike ohlotsheni lwamangqamuzana okuba yisiko, ivolumu yesiko efiselekayo, kanye nenhloso yezivivinyo ezahlukahlukene.

1. Umehluko kanye nokukhethwa kwephansi phansi kanye ne-Round Forset (ama-U-Shape ne-V-Shaped) amapuleti.

Amapuleti amasiko aphansi aphansi ngokuvamile asetshenziselwa amaseli anamathela;

Uhlobo v lusetshenziselwa isiko lokumiswa kwamaseli;

Amapuleti amasiko abunjiwe e-U asetshenziselwa kakhulu ukusikwa amaseli okumiswa;

Uhlobo lwe-V amapuleti kwesinye isikhathi lusetshenziswa ekuhlolweni kwe-hemaglutination ye-immunlological.

Izinhlobo ezahlukahlukene zamapuleti amasiko zisebenza ngezinhloso ezihlukile. Amangqamuzana amasiko ajwayele ukuba nefulethi eliphansi, elilungele ukubonwa kwesiMicroscope, indawo ephansi icacile, futhi ukuphakama kwesiko eliphakathi nendawo okuphakathi nendawo okungapheli, okubuye kulungele ukutholwa kwe-MTT. Ngakho-ke, noma ngabe amaseli anamathela noma amaseli okumiswa, izivivinyo ezinjenge-MTT ngokuvamile zisebenzisa amapuleti aphansi aphansi. Ukumunca kumele kulinganiswe kusetshenziswa amapuleti amasiko aphansi.

Amapuleti abunjiwe noma abunjiwe e-V ngokuvamile asetshenziselwa ezinye izidingo ezikhethekile. Isibonelo, e-Immunology, lapho ama-lymphocyte amabili ahlukene axutshwa emasikweni, kufanele ahlangane nawo ukuze avuse. Njengamanje, amapuleti abunjwe nguwe asetshenziswa kakhulu ngoba amaseli ahlanganiswe endaweni encane ngamandla adonsela phansi.

Amapuleti amasiko aphansi ayindilinga abuye asetshenziselwa izivivinyo zokufakwa kwe-isotope ezidinga ukuvuna amaseli ngomvuli weseli, isib. B. "I-Lixed Lymphocyte Isiko", njll. Ama-V-Plutes asetshenziswa kakhulu ekubulaweni kweseli nokuhlolwa kwe-immunohemagglunination. Ukuhlolwa kokubulawa kweseli nakho kungathathelwa indawo amapuleti abunjiwe (centrifugation aphansi ngemuva ngemuva kokungeza amaseli).

Iningi lamasiko weseli lisebenzisa amapuleti amasiko aphansi, alungele ukubonwa kwesiMicroscope, indawo ephansi icacile, futhi izinga loketshezi lwesiko leseli lihlala lihlala lihlala likhona.

2. Umehluko phakathi kwepuleti lesiko leselula nepuleti le-microtiter

Amapuleti e-Elisa ngokuvamile abiza kakhulu kunamapuleti amasiko amaseli. Amapuleti asemasheya asetshenziselwa ikakhulukazi isiko leseli, futhi angasetshenziswa ukuthola ukugxila kwamaprotheni; Amapuleti e-Elisa afaka amapuleti ahlanganisiwe namapuleti okusabela, futhi ngokuvamile awasetshenziselwa isiko leselula. Zisetshenziswa ikakhulukazi ekuphendukeni okuxhunyaniswe nge-immunoenzyme. Ukutholwa kwamaprotheni okugcina kudinga izidingo eziphakeme kanye nezixazululo ezisebenzayo ze-enzymeled ezisebenzayo.

3. Indawo ephansi ngaphansi kwamapuleti amasiko ahlukahlukene asetshenziswa kakhulu kanye nenani elinconyiwe loketshezi oluzongezwa

Ileveli ye-Liquid yesiko eliphakathi kwamapuleti e-orifice ahlukene akufanele lijule kakhulu, ngokuvamile enanini le-2-3 mm, futhi inani elifanele lentengo elizofakwa kwisiko ngalinye kahle lingabalwa ngokususelwa endaweni ye phansi kwepuleti le-orifice. Entwasahlobo ehlukile. Uma inani le-ketshezi elengezwa liningi kakhulu, lizothinta ukushintshaniswa kwegesi (i-oxygen Exchange), futhi kulula ukuchichima futhi kubangele ukungcoliswa ngesikhathi sokuhamba. I-cell cell density ingezwe ingalawulwa kalula ngokwenhloso yokuhlolwa. Ipuleti le-Yongyue Medical Cell Culture lenziwe nge-polystrerere (PS), ekhiqizwa emhlanganweni wokuhlanza osezingeni eliphansi, inzalo nge-irradiation, ayinayo i-dnabiation, i-rnase, akukho pyrogen, ephephile futhi inobungane. I-Yongle Culture Plate inemininingwane ehlukahlukene yokuhlangabezana nezidingo zama-bacterial ahlukene amagciwane.

I-Yongyue Medical, umenzi oma-munye wokudla kwelebhu kanye nama-reagents, kufanelekile ukwethembana nokukhetha kwakho!

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