Ukuchazwa okuningiliziwe kokuguquguquka kwe-reagent kuzivivinyo ze-PCR

Ukuchazwa okuningiliziwe kokuguquguquka kwe-reagent kuzivivinyo ze-PCR.

Ku-PCR Experiment, ngemuva kokungeza uhlelo lokuphendula, simboza ngokucophelela isivalo futhi siphinde siqinisekise ukuthi isivalo sivaliwe ukugwema ukuguquguquka kwe-realent. Kepha kwesinye isikhathi sibuye sibe nokungabaza: Sikuqinisekisile ukuthi isivimbo sivaliwe ngokuqinile, kodwa ngemuva kokuqedwa kokuhlolwa, i-reagent izovila. Siyini isizathu?

Eqinisweni, ukuguquguquka kwe-realent kungenye yezinkinga ezivame kakhulu ekuhlolweni kwe-PCR, futhi kunezinto eziningi ezithinta ukuguquguquka kwe-realent.

Ukuhanjiswa komhlaba kubhekisele kulokho ngesikhathi sokuhlolwa kwe-PCR, ikhambi elisheshayo liphuma emazingeni okushisa aphezulu, bese livumelana nodonga lwebhodlela noma amaconsi aphezulu ukuze akha igebe lamanzi noma amafilimu aphezulu, okuholela eshintsheni ekuqoqweni kwekhambi lokuphendula. Izinguquko, inani lekhambi lokuphendula lehlile, futhi abanye bavele bavele bavele bomile, okuholela ekuhlolweni okungalungile.

1. Ukucindezela kwe-Hot Lid, izinga lokushisa

Kuzihloli ze-PCR, imvamisa sishisa isembozo ukuvimbela ukubekezelela komoya we-realent. Izinga lokushisa lesembozo elifudumele livame kakhulu ukwedlula leso sixazululo sokusabela ukuvikela ukubekezelela ama-vapors.

I-Thermal Cycler Heating Lids ingahlukaniswa cishe ngezinhlobo ezine ezilandelayo: Amabhodlela okushisa angaguquki, amabhodlela okushisa aguqukayo, amabhodlela okushisa avumelanayo, namabhodlela wokushisa azenzakalelayo. Isembozo esifudumele esiguqukayo sibonisa ukucindezelwa okuqinile ngomunwe. Uma kungaqiniswa ngokuqinile, ama-reagents azoshintsha. Ukuhwamuka kwama-Edge kungancishiswa ngokwandisa ingcindezi kwisembozo esifudumele. Kwesinye isikhathi ukuqeda ukucindezela kungacindezela ishubhu bese kusonta ifindo. Ngaphansi kwezimo ezijwayelekile, isembozo sokufudumeza sikagesi singabona ngokuzenzakalelayo okuhlaziyi okuhlukile, kucindezela ngokuzenzakalelayo, futhi ingcindezi ifana ne-realegent from kanye namaphutha wokuhlola, kanye nokwenza ngcono ukunemba nokuphindaphinda.

2. Ukuchazwa kwezinto zokudla

Ngokuvuza okuvuzayo, iningi labantu licabanga ukuthi ingcindezi yevivo elishisayo ayanele. Ingcindezi yesembozo esishisayo ibaluleke kakhulu, kepha yileso sinye kuphela. Eqinisweni, izifebe zibaluleke kakhulu.

Amapuleti e-PCR e-Orifice ajwayele ukwenziwa ngezinto zokwakha zepulasitiki, ezikhula futhi zikhanye kakhulu ngemuva kokufudunyezwa. Izinga lokunwebeka kwe-thermal lesisekelo sensimbi se-PCR esetshenziselwa ukubeka ipuleti ye-PCR Orifice liphansi kakhulu, futhi ipuleti le-PCR lase-Orifice alikwazi ukwandisa ngokujwayelekile endizeni ngemuva kokwandisa okushisayo. Umbuso we-bulting wenza isembozo sokushisa esiphezulu singakwazi ukuxhumana ngokuphelele nendawo yepuleti ye-PCR ye-Orifice, okuholela ekushiseni okungalingani nengcindezelo, okwenza ukuhwamuka kwe-realent emaphethelweni weplanethi ye-PCR ye-Orifice, futhi kuthinte umphumela wokutholwa kwe-PCR.

3. Izifeni azivaliwe kahle

Ukuqina kwesembozo se-thermal kanye nokudla akusho ukuthi i-PCR TUBE CAC ivalwe ngokuqinile, futhi ikhwalithi yepuleti le-PCR kahle alilingani. Ukudla okuseqophelweni eliphezulu, ingaphezulu le-nozzle nesembozo kubushelelezi, futhi uphawu ngemuva kwebhokisi lesembozo kulungile, futhi akulula ukuvuza. Ukudla okuphansi kunezikhala ezingalingani kanye nemigqa ye-radial, engadala kalula ukuvuza noma ukuyomile.

Ngakho-ke, kwizibuko ze-PCR, zama ukukhetha okuhlangenwe nakho okuseqophelweni eliphezulu nefanele. Ngasikhathi sinye, uhlelo lokuphendula lunganwetshwa ukuze unciphise ithonya lokuhwamuka kohlelo ekuphenduleni.