Amapuleti e-Elisa for Elisa Assay Kit

Ngeza Ukulawulwa: Faka imithombo emi-3 yokulawula okungalungile, 1 Well okulawulwa okuhle, kanye ne-100Ul ye-serum yokulawula.
Ukufuhlazeka: Ukusabela e-ELISA 96 kahle ipuleti kudinga ukunyakaziswa ukwenza isampula lixutshwe ngokuphelele, bese lifakwa ku-37 ° C incubator noma amanzi okugeza amanzi imizuzu engama-20 yokusabela.
Geza ipuleti le-ELISA: Giniza isixazululo sokuwasha 15m ngamanzi afakwe emanzini aphukile kumthamo we-300ml. Lapho ukugeza izandla, okuqukethwe emithonjeni yepuleti yokusabela kufanele kuthululelwe, isixazululo sokuwasha kufanele sigcwaliswe esikhundleni semithombo yokusabela, bese sibekwe imizuzu engama-30 bese sinyakazisa ngamandla. Phinda lesi senzo izikhathi ezi-5 bese uqala ukuyomile. Ukugeza ngomshini kudinga ukwenziwa izikhathi ezi-5, umgodi ngamunye ufakwa nge-200U yesisombululo sokuwasha noma ungagcwaliswa, bese uyeka
Ngemuva kwemizuzu engama-30, chitha futhi usome.
Faka i-Enzyme Label Working Solution: engeza i-100el ye-Enzyme ilebula Isixazululo Sokusebenza Kokunye, uyibeke ku-incubator ku-37, bese ugeza amahlaba amahlandla ama-5 ngemuva kwemizuzu engama-20 yokusabela. Ukusebenza kokugeza i-Elisa Microplate kuyafana nezinyathelo ezingenhla.
Lesi yisinyathelo sokuqala sokusabela, okuthuthukiswa kombala kanye nokuqedwa kokusabela: 50Ul of the substrate ingezwe ekuphenduleni kahle, kanti umbala wenzelwe imizuzu eyi-10 ebumnyameni ngaphansi kwezimo ezingama-37. Faka isixazululo STOP kumthombo ngamunye
U-50UL wahlanganiswa nokumisa ukusabela.
Ukusetshenziswa kwamapuleti ama-runsen microtiter kudinga ukwenziwa ngaphansi kwezimo eziyinyumba. Ukubulala amagciwane nokuhlanza kufanele kwenziwe ngokucophelela ngemuva kokusebenzisa ipuleti le-microtiter, kepha kufanele kuqashelwe ukuthi amanye ama-enzyme
Ngaphambi kokubulala amagciwane ebhodini elijwayelekile, udinga ukufunda imiyalo ngokucophelela ukuze ubone ukuthi ingafakwa inzalo emazingeni okushisa aphezulu.