Amapuleti we-cell kanye nezicubu zamapuleti ku-microbiology

I-Yongyue Medical Gonts igxile ekuhlolweni kwesiko leselula. Njengethuluzi elijwayelekile nelibalulekile lamaseli, amapuleti amasiko amaseli anezimo ezahlukahlukene, imininingwane nokusetshenziswa. Ukukhethwa kwamapuleti ama-Cell Culture: 1. Amapuleti amasiko amaseli angahlukaniswa ngamapuleti aphansi aphansi kanye namapuleti aphansi azungeze (akhiwe futhi abunjiwe futhi anjwe v) ngokuya ngesimo esingezansi; 2. Inani lemithombo yesiko lingu-6, 12, 24, 48, 96, 384, 1536 Wells nokunye; Amakhasimende angazikhethela amapuleti abo esiko ngokuya ngezidingo zawo zokuhlola. Kuyiqiniso, siphinde sibe nezitsha ze-petri, amasiko, ama-pasteur Pipette, acindezele ama-hanger, njll. Ukufeza izivivinyo zesiko leselula. Uma udinga insiza eyenziwe ngokwezifiso, sicela uxhumane nomnyango wethu wokuthengisa.

Sihlukanisa futhi sikhethe phakathi kwamapuleti amasiko aphansi nangaphansi okuyindilinga (okwenziwe nge-U-Shape ne-V)

1) Amapuleti amasiko anokuphansi okuphansi ngokuvamile asetshenziselwa amaseli anamathela.

2) Uhlobo lwe-V ngokuvamile lusetshenziselwa isiko lokumiswa kwamaseli.

3) Amapuleti amasiko amise okwenziwe ngo-U nawo asetshenziselwa isiko lokumiswa kwamaseli.

4) Amapuleti amasiko abunjiwe e-V kwesinye isikhathi asetshenziselwa izivivinyo ze-hemagglunination ammeng.

Imininingwane engaphezulu

Ezinye izithombe

Umbuzo: Ngiyabona ukuthi ipuleti lesiko linokucaciswa okuningana, okungukuthi 4, 6, 12, 24, 48 no-96 Wells, kodwa angazi ukuthi yiluphi uhlobo lokuhlola, ngicela ululeke.

Impendulo: Ngokuya ngezidingo zakho ezithile zokuhlola, i-cytometry yokugeleza imvamisa isebenzisa ipuleti lesiko le-6, i-MTT livame ukusebenzisa ipuleti lesiko le-96 kahle, isiko leseli livame ukusebenzisa ipuleti lesiko le-24, njll! Kuya ngokuhlola kwakho!

Umbuzo: Uyini umehluko phakathi kwepuleti ye-microtiter nepuleti lesiko leselula?

Impendulo: Amapuleti e-ELISA ngokuvamile abiza kakhulu kunamapuleti amasiko amaseli. Amapuleti ama-Cell Culture asetshenziselwa amasiko weseli, kepha futhi angasetshenziswa ukukala okuhlushwa amaprotheni; Amapuleti e-Elisa afaka amapuleti okuhlanganisa kanye namapuleti okusabela, ngokuvamile akwazi ukusetshenziselwa isiko leseli. Zisetshenziselwa ikakhulukazi ama-cell cellel cound.protein ukutholwa ngemuva kokusabela kwe-immunoenzyme chain ukusabela kudinga izidingo eziphakeme kanye nesixazululo esithile sokusebenza se-enzyme esibhalwe phansi.