Amapuleti e-PCR akhiqizwa yi-yongyue Medical ngokuvamile ahlukaniswe ngamapuleti e-PCR angenamikhawulo, amapuleti we-PCR esiketiwe ama-PCR anesiketi esigcwele. Amapuleti ngaphandle kwesiketi: Iyahambisana nabakhi be-Thermal abaningi noma imishini ye-QPCR, kepha ayilungele izinhlelo zokusebenza ezenzakalelayo. Ukuqina akuphezulu ngesikhathi sokuphamba, futhi kufanele zisetshenziswe nomnikazi wepuleti. Ipuleti le-semi skirtrid PRCR: Lingashintshwa ukuze ilebula noma uhlelo lwebhakhodi, ilungele uhlelo lokusebenza oluzenzakalelayo futhi inokuqina okuhle kwepayiphu. Iplanethi ephelele ye-STITT PCR: Ilungele kahle izinhlelo zokusebenza zelebhu ezenzakalelayo, kepha futhi ilungele amalebula nezinhlelo zokusebenza zebhakhodi. Amandla amahle emishini, alungele amathuluzi we-PCR anamamojula wokugcina, kanye nokuqina okuphezulu ngesikhathi sokuphamba. Ngokwe-96 Well Plate Plate noma ipuleti lika-384 Well PRCR, amakona okusika kanye namalebula asuselwa kwimisebenzi yamakhona namalebula. Ukusika amakhona: Ukukhethwa kwesikhundla sokusikwa ekhoneni kwepuleti ye-PCR kuncike kwizidingo zensimbi elihlanganisiwe, ezingabekwa kahle.

Ukukhonjwa: Ukulebula kwe-alphanumeric kwamapuleti e-PCR kusiza ukukhomba imithombo ngayinye nezindawo ezisampula. Lokhu kuvame ukuba yilogo enemibala enemibala noma uphawu oluqoshiwe. Kwezinye izivivinyo zohlelo lokusebenza ezenzakalelayo, ipuleti yokuphendula livalwe kangcono ngokumaka okuphrintiwe.

①Kungani yizicoco ze-PRR ngokuvamile ezenziwe nge-PP?

Ngoba okufekezwayo kwe-PCR / QPR ngokuvamile kuthintana ngqo nama-reagents noma amasampula, ne-polypropylene (PP) kungukuqhathaniswa okuhle kwama-biomolecule futhi kunokuvuselelwa kwezinga lokushisa noma ukubekelwa phansi kwe-121 ° C futhi uphinde ubekezelele ukuguquguquka kokushisa ngesikhathi sokushisa ngokushisa).

Uma ivolumu yesampula incane, kukhethwa ishubhu elilodwa noma intambo yeshubhu. Kodwa-ke, lapho kudingeka ivolumu enkulu yokuphendula, ishubhu elilodwa liyavela eProuble, futhi ivolumu ingaba ngu-0.5 ml. Isembozo esicaba kanye nesembozo se-convex zinezinzuzo zazo. Sicela ubheke imininingwane elandelayo ngemininingwane.

I-tube eyodwa: 0.2ml noma i-0.5ml, ukukhetha okuguquguqukayo kwe-Samplemu Volume.0.2 ML PCR Tubes.0.5 ML PCR TUBES.

Amashubhu e-PCR: 0.2ML noma 0.15ml, 8 strip PCR run noma amashubhu ayi-12

I-FLAT Cap: Inikeza ukuhanjiswa kwesiginali okunembile kwe-fluorescent kwe-QPCR futhi kusiza ukubhala nokubhala

Isembozo seConvex: Ixhumana nesembozo esishisayo sensimbi ye-PCR ukwehlisa ukonakala kweshubhu reaction ngaphansi kwengcindezi; Kepha kuthinta ukuhanjiswa kwezimpawu ze-fluorescent futhi akunakusetshenziswa ku-QPCR.

② Kungani eminye imithombo ye-PCR Plate Wells iphuma futhi eminye icaba? Yikuphi okungcono?

Lowo ofanelekile kakhulu. Kunezinhlobo ezimbili zamapuleti ajwayelekile we-PCR: imiphetho emithombo efulethini futhi aphakamise imiphetho kahle. Amapuleti anemiphetho kahle egcwele amakhilomitha amaningi ama-thermal. I-Plate Plate Seals emiphethweni kahle yokubekwa uphawu kalula, ukunciphisa ubungozi bokungcoliswa kokuwela phakathi kwamasampula.

③ Ungayikhetha kanjani amashubhu we-PCR / amapuleti ngamakhono ahlukene?

Inhloso yokukhetha: Ukukhetha umkhiqizo ofanele ngokuya ngezidingo ezithile zokuhlola. Umthamo wamashubhu amaningi we-PCR angahlangabezana nezidingo zokuphendula kwe-PCR. Ngokusekelwe kulokhu, kunconyelwa ukusebenzisa amashubhu amancane wevolumu kuqala. Ukuthuthuka okuthuthukile okushisayo nokuhanjiswa okuncishisiwe ngenxa yekhanda elincane le-headpar yamashubhu wevolumu ephansi / amapuleti. Ngasikhathi sinye, ukunakekelwa kufanele kuthathwe ukugwema ukungeza ngokweqile noma okuncane kakhulu lapho ungeza amasampula. Kuningi kakhulu kungaholela ekulahlekelweni kokuvuselelwa okushisayo, ukuchitheka nokungcola, ngenkathi kuncane kakhulu kungaholela ekulahlekelweni kwesampula ngokuhwamuka.

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